seqcraft.onrender.com
Inspect plasmids, simulate PCR and restriction digests, compare constructs, and prepare cloning proposals in one exact, private workspace.
First seen 2026-09-14 · Last seen 2026-09-14 · Source: webmcp.com
Answer 14 · Act 42 · Transact 1 · Score: Not yet scored
Tool inspector
seqcraft_simulate_golden_gate
Simulate Golden Gate multi-fragment assembly using Type IIS restriction enzymes (e.g. BsaI, BsmBI, AarI). Validates compatible overhang pairs, checks junction fidelity, and returns the assembled construct.
Input schema
| Property | Type | Required | Description |
|---|---|---|---|
documentIds |
array | yes | List of document IDs participating in the assembly in order. |
enzymeName |
string | no | Type IIS enzyme driving the assembly (default: BsaI). |
Raw JSON schema
{
"type": "object",
"properties": {
"documentIds": {
"type": "array",
"items": {
"type": "string"
},
"description": "List of document IDs participating in the assembly in order."
},
"enzymeName": {
"type": "string",
"enum": [
"BsaI",
"BsmBI",
"BbsI",
"AarI",
"SapI",
"PaqCI",
"Esp3I"
],
"description": "Type IIS enzyme driving the assembly (default: BsaI)."
}
},
"required": [
"documentIds"
],
"additionalProperties": false
}
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