seqcraft.onrender.com

{
  "type": "object",
  "properties": {
    "mode": {
      "type": "string",
      "enum": [
        "pcr",
        "digest"
      ],
      "description": "Protocol type to generate (pcr or digest)."
    },
    "robotModel": {
      "type": "string",
      "enum": [
        "OT-2",
        "Flex"
      ],
      "description": "Target Opentrons robot model (default: OT-2)."
    },
    "numReactions": {
      "type": "integer",
      "minimum": 1,
      "maximum": 96,
      "description": "Number of reactions to prepare (1-96)."
    },
    "reactionVolumeUl": {
      "type": "number",
      "minimum": 5,
      "maximum": 100,
      "description": "Total reaction volume in microliters (default: 50)."
    },
    "documentId": {
      "type": "string",
      "description": "Target document ID. If omitted, uses active document."
    },
    "forwardPrimer": {
      "type": "string",
      "description": "Forward primer name (for PCR mode)."
    },
    "reversePrimer": {
      "type": "string",
      "description": "Reverse primer name (for PCR mode)."
    },
    "annealingTempC": {
      "type": "number",
      "description": "PCR annealing temperature in Celsius."
    },
    "ampliconLengthBp": {
      "type": "integer",
      "description": "Expected PCR amplicon length in base pairs."
    },
    "enzymeNames": {
      "type": "array",
      "items": {
        "type": "string"
      },
      "description": "Restriction enzymes for digestion (for digest mode)."
    },
    "incubationTempC": {
      "type": "number",
      "description": "Incubation temperature in Celsius for digest (default: 37)."
    },
    "incubationTimeMin": {
      "type": "integer",
      "description": "Incubation duration in minutes (default: 60)."
    }
  },
  "required": [
    "mode"
  ],
  "additionalProperties": false
}