seqcraft.onrender.com
Inspect plasmids, simulate PCR and restriction digests, compare constructs, and prepare cloning proposals in one exact, private workspace.
First seen 2026-09-14 · Last seen 2026-09-14 · Source: webmcp.com
Answer 14 · Act 42 · Transact 1 · Score: Not yet scored
Tool inspector
seqcraft_find_crispr_targets
Scan sequence for CRISPR-Cas guide RNA target sites and protospacers across multiple Cas effectors (SpCas9, SaCas9, Cas12a/Cpf1, Cas12e/CasX). Computes on-target quality scores, GC balance, Pol III termination penalties, and MMEJ frameshift predictions.
Input schema
| Property | Type | Required | Description |
|---|---|---|---|
documentId |
string | no | Document identifier. If omitted, uses active document. |
nuclease |
string | no | CRISPR Cas nuclease system (default: SpCas9). SpCas9 (3' NGG), SaCas9 (3' NNGRRT), Cas12a (5' TTTV), Cas12e (5' TTCN). |
minQualityScore |
integer | no | Minimum quality score threshold (0-100, default: 0). |
maxResults |
integer | no | Maximum number of target sites returned (default: 20). |
Raw JSON schema
{
"type": "object",
"properties": {
"documentId": {
"type": "string",
"description": "Document identifier. If omitted, uses active document."
},
"nuclease": {
"type": "string",
"enum": [
"SpCas9",
"SaCas9",
"Cas12a",
"Cas12e"
],
"description": "CRISPR Cas nuclease system (default: SpCas9). SpCas9 (3' NGG), SaCas9 (3' NNGRRT), Cas12a (5' TTTV), Cas12e (5' TTCN)."
},
"minQualityScore": {
"type": "integer",
"minimum": 0,
"maximum": 100,
"description": "Minimum quality score threshold (0-100, default: 0)."
},
"maxResults": {
"type": "integer",
"minimum": 1,
"maximum": 100,
"description": "Maximum number of target sites returned (default: 20)."
}
},
"additionalProperties": false
}
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